Drug Discov Ther. 2022;16(3):135-138. (DOI: 10.5582/ddt.2022.01040)

Proposal of an in vitro thrombus-growth model for evaluating anticoagulants

Sawaguchi Y, Yamamoto H, Itou S, Tachibana K, Ohnuma K, Kamada Y, Nakajima T


The general anticoagulant evaluation requires high expense equipment, reagents, and space. Therefore, not all laboratories can perform research related to anticoagulant. In this study, we propose a novel simple method "in vitro thrombus-growth model" that can evaluate anticoagulant ability by measuring weight. The in vitro thrombus-growth model is prepared by creating a "growthclot" with citrate plasma, calcium chloride, and thrombin, and then pouring new citrate plasma onto it. The prepared growth-clots were increased in volume in citrated human plasma, including surpluses calcium chloride, which was released slowly, leading to clot coagulation around the plasma. As a result of evaluating the anticoagulant ability of direct thrombin inhibitor using this in vitro thrombus-growth model, it was confirmed that clot growth was suppressed in a concentrationdependent manner. Therefore, this thrombus-growth model is useful as a primary anticoagulant test that can to discover compounds with anticoagulant activity perform in any laboratory.

KEYWORDS: Thrombus-growth model, anticoagulants, thrombosis, fibrin clot, in vitro model

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