Drug Discov Ther. 2021;15(5):268-272. (DOI: 10.5582/ddt.2021.01092)

Antiviral effect of electrolyzed reduced water on SARS-CoV-2

Okajima Y, Okajima M, Ikeda M, Wada Y, Shimokawa K, Ishii F


The inhibitory activity of electrolyzed reduced water (ERW) against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which is the etiological agent responsible for coronavirus disease 2019 (COVID-19), was tested in vitro on Vero E6 cells using a plaque assay. Infectious virus titers of cells treated with ERW 100%, 50% and 33.3% solutions and phosphate buffered saline (PBS, negative control) and exposed to the virus suspension for 60 seconds were 2.25, 2.65, 3.21 and 7.38, respectively. ERW has a high pH and low surface tension. It is considered that the alkaline property of ERW breaks down phospholipids and proteins of envelopes. The role of pH and reducibility on the virucidal effect of ERW should be further evaluated. This study provides a foundation for utilizing ERW as an effective antiviral aqueous solution in a variety of applications.

KEYWORDS: Electrolyzed reduced water, alkaline electrolyzed water, reducibility, SARS-CoV-2, antiviral activity

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